Increasing pregnancy rates have been achieved by using the latest techniques developed with embryo freezing and thawing processes, which have been in use for about 25 years.
Instead of the slow freezing technique, vitrification (fast freezing) technique, which is now much more successful, is used. The embryos are stored in liquid nitrogen at -196 ° C in the sleep state by cryoprotectant substances. When the transfer time comes, they are removed from the nitrogen and the procedures are reversed and the transfer is prepared.
In our centre, embryos are generally frozen on the 3rd and 5th day.
According to the statistics, the better the quality of the frozen embryos, the chances of survival during thawing and obtaining a positive pregnancy result rate are higher.
In the future to transfer frozen embryos in Gyno Life IVF Centre provides a chance for couples to retry with much lower costs. Additionally, there is no need for long-term medication. The embryo freezing and thawing is easier, shorter, and less of an expense method which offers almost the same chances of a pregnancy compared with the transfer of fresh embryos.
If there are enough numbers and quality of embryos, embryo freezing is done by taking the consent of the participating couple. The first benefit is that if the pregnancy does not occur in the first application, it allows embryo transfer without ovulation induction. Thus, medication is not used and egg collection is not done.
We know that egg quality deteriorates with age. A woman who has previously frozen embryos will be able to take them after a few years. The possibility of pregnancy from freezing and thawed embryos is close to the chance of pregnancy from fresh embryos in our fully equipped IVF centre in Cyprus.
For this, embryo freezing and thawing are done with great care. The intensities of the freezing liquid used is made to be increased gradually. With the help of these fluids, the water in the embryo is slowly removed and replaced by a substance that does not expand during freezing and therefore does not cause the membrane to break down.
This stage is done at a certain temperature and in a gradual manner, so as to give the embryo the least damage. When the waiting period is complete, the tube or freezing instrument containing the embryos is placed in the freezer area. The quality and capacity of this device is also very important for the healthy freezing.
The freezing program is determined by the embryologist and saved in the device, the program is started accordingly. In principle, the temperature is gradually reduced to -30ºC. Thereafter, it is reduced to -150 ° C more rapidly and frozen embryos are placed in storage vessels which contain liquid nitrogen and embryos are stored at -180° C.
No devices are used during thawing. The chemical liquids used during freezing are then prepared in reverse order. The embryos coming to the room temperature are taken to the most concentrated liquid. Waiting times are monitored carefully and embryos which are brought to body temperature of 37ºC again are placed in gaseous, moist, hot incubator.
Depending on the day and the number of days they are frozen, they are either transferred on the same day or by observing for a couple of days the good quality embryos that continue to divide are selected. Usually this route is preferred.